周庭銀 趙虎 賀偉 作者單位：上海長征醫院
（潘云華供稿）
[摘要] 目的 評價ESP全自動快速血培養系統的功能及影響因素。 方法 應用ESP檢測臨床標本416份（普通血培養388份，痰結核培養28份），觀察其陽性檢出率、檢出時間，并對所檢出的病原菌進行分析。觀察不同細菌濃度、溫度和放置時間，以及抗生素對檢測結果的影響。 結果 388份血培養中檢測出陽性標本68株，陽性率為17.3%,假陽性為1.3％，其中24小時內檢出67.6%，48小時內檢出25％，48小時后檢出7.4％。分離出病原菌23個種屬。檢測速度與細菌濃度成正比；一定量的菌液濃度加入培養瓶內，放置機外不同濕度、不同時間對陽性檢出率影響不大，但總的報告時間延長；培養瓶可以中和一定濃度的抗生素。28份抗酸染色陽性的痰液標本，ESP檢出結核桿菌25份，與常規培養一致，最快檢測時間4.74天，最慢30.93天，醫療器械平均為15.9天。結論 ESP儀是一種具有多功能的自動化程度高、快速、正確等優點的培養系統。
[關鍵詞] 血培養；結核菌培養；ESP系統
Clinical Evaluation Of Difco ESP Blood Culture System Zhou Tingyin, Zhao Hu, He Wei. Shanghai Chang-Zheng Hospital, Shanghai 200040.
[Abstract] Objective To Evaluate Difco ESP Blood Culture System And Analyze The Positive Rate Effected By Different Factors. Methods 416 Clincal Specimens (Blood Specimen 388 Sets, Suspicious TB Sputum Specimens 28 Sets) Were Incubated By ESP. The Positive Rate, Speed, False Positive Rate And The Influent Factors Were Observed And Analyzed. Results In A Total Of 388 Blood Culture Sets, 68 Cases Were Positive (17.3%). Among Them, 46 Cases (67.6%) Positive In Less Than 24 Hours, 17 Cases (25%) Positive During 24 (48 Hours), And 5 Cases (7.4%) Positive Over 48 Hours. The Incubate Speed Had Good Relationship With Bacterial Concentration In The Specimens. The Positive Rate Was Not Effected When The Culture Bottles Hold In Different Time And Temperature In Vitro. And The Antibiotics In The Specimens Could Be Neutralized By The Culture Bottle. In The Suspicious TB Sputum Specimens, 25 Cases Were Positive, As The Same With The Routine Culture Result. The Fastest Incubation Time Of TB Was 4.74 Days, And The Slowest Time Was 30.93 Days. Conclusion Difco ESP Blood Culture System Was A Speedy, Reliable, And Multi-Function Culture System.
[Key Words] Blood Culture; Mycobacterium Culture; ESP
近年來，自動化非介人性連續瓶外檢測的血培養儀已逐漸在國內推廣使用，主要機型有Difco公司ESP系統、BD公司的Bactec9000系統和生物梅里埃公司的VITAL系統等，對提高血培養的陽性率，快速準確地作出病原學診斷提供了十分有利的條件。我們引進美國Difco公司研制的最新產品ESP全自動多功能培養系統已近一年，現就臨床應用方面的評價報告如下。